In this screening study we are reporting antibacterial effect of ethanol extract of Anatolian propolis on Helicobacter pylori. For this five different strains of Helicobacter pylori (HP1, HP2, HP5, HP9 and HP13) were isolated by endoscopic indications and fifteen different ethanolic extracts were used to evaluate their antimicrobial activities. J99 strains of the bacteria was also obtained from Refik Saydam Institutes and the results were compared with them. Inhibition effects of the samples on Helicobacter pylori urease were also investigated. All propolis extracts showed antimicrobial effects against the H. pylori at different zone diameters, ranged from 12 to 47 mm. All samples also showed inhibition for J99 bacteria urease enzyme at different concentration, IC50 were changed from 0.26 to 1.53 mg/mL. In conclusion, ethanolic propolis extracts have highly effective potential against the bacteria, but types of propolis is a key factor in it.